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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with <t>DAPI</t> ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
Vectashield With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with <t>DAPI</t> ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with <t>DAPI</t> ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with <t>DAPI</t> ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
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vectashield hardset antifade mounting medium with dapi  (Vector Laboratories)
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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with <t>DAPI</t> ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
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https://www.bioz.com/result/vectashield hardset antifade mounting medium with dapi/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with <t>DAPI</t> ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with <t>DAPI</t> ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
Dapi Counterstain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with DAPI ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).

Journal: European Journal of Clinical Investigation

Article Title: FNDC4 and FNDC5 Attenuate SARS‐CoV‐2 S1‐Induced Inflammatory Responses in Human Adipose Tissue

doi: 10.1111/eci.70215

Figure Lengend Snippet: Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with DAPI ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).

Article Snippet: After washing, coverslips were mounted using Vectashield with DAPI (H‐1500‐10, Vector Laboratories, Burlingame, CA, USA) for nuclear counterstaining.

Techniques: Expressing, Immunofluorescence, Marker, Staining, Immunohistochemical staining